Abstract: Abstract Based on the microwave attenuation theory of weakly ionized dusty plasmas, an experimental study of microwave transmission in weakly ionized dusty plasma was carried out. A vector network analyzer was used to record the transmission attenuation of 4-6 GHz microwaves in weakly ionized dusty plasmas. The experimental results are in good agreement with the calculation results of the weakly ionized dusty plasmas microwave attenuation theory, which further verifies the reliability of the theory. The verification of the theory is of great significance to establish a new method for measuring the internal parameters of the weakly ionized dusty plasmas.