Abstract: Pseudorange bias is an important error source to restrain the improvement of BeiDou Navigation Satellite System(BDS) service capability, which affects users' real-time positioning performance and satellite precise orbit determination. However, there are few researches and measurements on the pseudorange bias of the BDS-3, this paper theoretically analyzes the influence of front-end bandwidth and correlator spacing on pseudorange biases of receivers, and simulates the theoretical analysis results. Then on this basis, the live signals of the BDS-3 was collected with the 7.5 m dish antenna, and the pseudorange biases of the measured data of the live satellite was tested and verified by the software receiver, comprehensively analyzing the influence of front-end bandwidth and correlator spacing on the pseudorange bias of the BDS-3 satellite signal. According to the results of theoretical analysis and test verification, the influence range of front-end bandwidth and correlator spacing of the receiver on the pseudorange bias of the BDS-3 satellite B1I B3I and B1C is obtained. The pseudorange bias of BDS-3 satellite signal can be effectively reduced and the positioning accuracy of users can be improved by setting the suggested parameters.