Abstract: The Rydberg atoms can achieve high sensitivity detection of microwave signals using electromagnetic induced transparency effects, and the Rydberg atomic superheterodyne receiver has been widely studied in wireless communication, detection, and other applications in recent years. However, intentional or unintentional microwave pulse interference is difficult to avoid in real physical environments. Currently, the impact of microwave pulse interference on Rydberg atoms in typical radar detection applications is not clear, such as moving target detection(MTD). In this work, the impact of microwave interference on the receiving characteristics of the Rydberg atomic superheterodyne receiver has been theoretically analyzed at first. Then, an experimental system is designed and the MTD function of Rydberg atoms under microwave pulse interference is implemented. It is proved that Rydberg atoms can accurately measure the speed of moving targets. Subsequently, the MTD performance of Rydberg atoms under different intensities of microwave pulse interference and laser intensity is studied, and relevant theoretical explanations are provided. Analysis shows that using strong probe or coupling light within the conventional laser intensity range can effectively enhance the anti-blocking ability of Rydberg atoms.